Fig. 3

Tapering of bleomycin (bleo)-induced TRIM72 upregulation correlates with an increase in lung collagen content. a H&E and Masson’s trichrome staining on lung sections from 5 to 6-month-old B6 WT mice received i.t. of 75 μl 1.5 U/kg bleo. Tissues were harvested at days 0, 7, 14, and 21 after i.t. respectively. Scale bar = 100 μm; b α-smooth muscle actin immunostaining (α-SMA, green) on bleo-treated lung, counter-stained with DAPI (blue). Red arrow = fibrotic area. Scale bar = 50 μm; c hydroxyproline content per right lung after bleo i.t.; d Western blot of TRIM72 in bleo-treated WT lungs at the above time points; e quantification of TRIM72 protein and Trim72 mRNA in mouse lung samples. n = 4 for D0 and D7, n = 6 for D14 and D21, *P < 0.05 or **P < 0.01 compared to day 0 based on one-way ANOVA with post hoc analysis