Fig. 2

Increased NLRP3 inflammasome activity in miR-223^−/− mice during neutrophilic asthma. a Proteins expression of NLRP3, ASC, caspase-1, pro-caspase-1 and GAPDH were measured by western blot. Representative immunoblot from three independent mice. b Proteins expression of IL-1β, pro-IL-1β, IL-18, pro-IL-18 and GAPDH were measured by western blot. Representative immunoblot from three independent mice. n = 6–8 mice/group; statistical significance was determined by ANOVA. c Genes expression of NLRP3, ASC, caspase-1, IL-1β and IL-18 were determined by qPCR. n = 6–8 mice/group; statistical significance was determined by ANOVA. All data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001