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Fig. 1 | Respiratory Research

Fig. 1

From: MiR-223 plays a protecting role in neutrophilic asthmatic mice through the inhibition of NLRP3 inflammasome

Fig. 1

MiR-223 played an important role in regulating neutrophilic airway inflammation in the OVA/CFA-induced asthma model. a Mice were sensitized against OVA in the presence of complete Freund’s adjuvant (CFA), followed by exposure to 1% OVA aerosols on days 21 and 22. Administration of miR-223 agomirs or negative control agomirs to OVA-sensitized mice for 3 consecutive days was shown. Analysis was performed at day 23. b MiR-223 expression levels were detected in the lung tissues from mice after OVA sensitization (1 day), after first challenged (22 day) and in asthma model (23 day) by qPCR, respectively. n = 6–8 mice/group; statistical significance was determined by ANOVA. c Representative micrographs of lung H&E staining a-d and PAS staining e-h from different groups (200× magnification). d, e Semi-quantification of lung inflammatory score and Apas+/Pbm was performed. n = 6–8 mice/group; statistical significance was determined by ANOVA. f Number of total inflammatory cells, neutrophils, eosinophils, and lymphocytes was calculated in BALF. n = 6–8 mice/group; statistical significance was determined by ANOVA. g AHR was determined by lung resistance. n = 6–8 mice/group. Statistical significance was determined by ANOVA. h-p The levels of Th2-associated cytokines (IL-4, IL-5, IL-13), Th17-associated cytokines (IL-17A, IL-22, IL-23), Th1-associated cytokine (IFN-γ), IL-1β, and IL-18 and in BALF were measured by ELISA. n = 6–8 mice/group; statistical significance was determined by ANOVA. All data were expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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