Fig. 1

Prolonged Scar-in-a-Jar as a model of fibrogenesis. Primary lung fibroblasts from healthy donors were cultured in the presence of ficoll to promote a crowded environment. Fibroblasts were stimulated with TGF-β1 and differentiated into myofibroblasts that produced high amounts of extracellular matrix proteins. Cells were cultured for 12 days with medium changes at day 4 and 8