MyD88 regulates a prolonged adaptation response to environmental dust exposure-induced lung disease

Johnson, Amber N.; Harkema, Jack R.; Nelson, Amy J.; Dickinson, John D.; Kalil, Julianna; Duryee, Michael J.; Thiele, Geoffrey M.; Kumar, Balawant; Singh, Amar B.; Gaurav, Rohit; Glover, Sarah C.; Tang, Ying; Romberger, Debra J.; Kielian, Tammy; Poole, Jill A.

doi:10.1186/s12931-020-01362-8

Respiratory Research

Table 1 Levels of mediators in lung tissue homogenates screened by treatment group

From: MyD88 regulates a prolonged adaptation response to environmental dust exposure-induced lung disease

Mediator	Action	WT Saline	WT ODE	KO Saline	KO ODE
CCL2	Attracts monocytes, memory T cells, DCs	181.5 ± 27.7	625.2 ± 18.4 **	371.1 ± 87.3	557.0 ± 96.0*
CCL3	Attracts neutrophils	21.5 ± 4.5	874.5 ± 198.3,###*	43.3 ± 11.9	100.4 ± 36.4*
CCL4	Attracts NK cells, monocytes	37.5 ± 5.3	140.6 ± 23.8, ###*	37.5 ± 3.7	48.8 ± 7.5
CCL5	Attracts T cells, eosinophils, basophils	252.1 ± 67.5	1410 ± 450.5**	545.1 ± 98.9	2519 ± 600.2*
CCL7	Attracts monocytes, activates MΦ	34.4 ± 9.2	552.5 ± 117.3,##*	27.5 ± 5.7	150.5 ± 53.7*
CCL8	Attracts mast cells, eosinophils and basophils, monocytes, T cells, NK cells	356.3 ± 163.3	1620 ± 620.6	591.8 ± 112.4	1181 ± 215.2
CCL11	Attracts eosinophils	94.2 ± 39.7	177.4 ± 52.3	77.8 ± 9.3	97.7 ± 10.7
CCL19	Attracts T and B cells	14.6 ± 2.5	18.7 ± 1.1	15.9 ± 1.4	22.2 ± 6.5
CCL22	Attracts Th2 and Treg	117.5 ± 38.3	432.1 ± 52.6**	208.5 ± 62.4	335.4 ± 88.3
CXCL1	Attracts neutrophils	62.3 ± 20.6	713.1 ± 211.3,##**	30.2 ± 6.2	47.8 ± 11.0
CXCL10	Attracts monocytes/macrophages, T cells, NK cells, DCs	44.2 ± 12.1	131.6 ± 29.3*	40.8 ± 3.1	107.2 ± 38.9
IL-4	Th2, allergy	236.2 ± 25.1	173.5 ± 16.3	303.9 ± 24.5	273.8 ± 5.1
IL-5	Eosinophil recruitment	3.4 ± 0.9	3.0 ± 0.2	2.6 ± 0.1	2.8 ± 0.1
IL-13	Regulates IgE, mucus	246.2 ± 14	173.5 ± 16.3	303.9 ± 24.5	273.8 ± 5.1
Periostin^a	Tissue remodeling	9.99 ± 1.11	21.14 ± 2.71, #*	8.91 ± 9.88	12.42 ± 1.40
MMP-12	Tissue remodeling	757.7 ± 337.9	13,351 ± 5086,##**	647.8 ± 133.5	854.5 ± 196.1
S100A8^a	Neutrophilic inflammation	229.9 ± 61.1	652.7 ± 66.4,###*	172.5 ± 33.8	215.4 ± 9.6
CHI3-L1^a	Inflammation and tissue remodeling	291.5 ± 42.1	*620.0 ± 9.5, #**	339.4 ± 67.6	468.9 ± 70.0
AREG	Tissue remodeling and repair	102.3 ± 11.6	221.8 ± 23.5**	122.4 ± 3.7	161.5 ± 19.0
Total Protein^a		7.50 ± 0.47	8.81 ± 0.41	6.47 ± 0.83	5.61 ± 1.46

Mean ± SEM values shown in pg/ml; except ^avalues are ng/ml. N = 4 mice/ group (2 males/2 females). Statistical difference denoted (*p < 0.05 **p < 0.01, ***p < 0.001) vs. saline. Statistical difference between WT ODE and KO ODE denoted (#p < 0.05, ##p < 0.01, ###p < 0.001). Differences are in bold

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