Fig. 4
From: MyD88 regulates a prolonged adaptation response to environmental dust exposure-induced lung disease
Mucous cell metaplasia is increased in MyD88 KO mice repetitively exposed to inhalant ODE. Wild-type (WT) and MyD88 knock-out (KO) mice were treated i.n. with saline or ODE daily for 3 weeks, whereupon lung tissues were collected, formalin-fixed, and paraffin embedded. Lung sections (5 μm) were PAS-stained for mucus cell determination with representative images for each treatment group shown (a). Key: b, bronchiolar airway; a, alveolar parenchyma; v = blood vessel; asterisk, lymphoid aggregates, and arrow denotes positive PAS stained mucus cells (scale bar is 200 μm). Lung sections were semi-quantitatively scored from 0 to 5 for mucus cell metaplasia with scatter plot (b) depicting means with SEM; N = 6 mice/group (N = 4 male, N = 2 female)