Fig. 7
Effect of a miR-451 inhibitor on the expression of MIF, vascular growth factors, IL-1β and IL-6. a Representative western blot showing the expression of MIF. b MIF expression quantified by densitometry with normalization to vinculin. Each column of the graph represents the mean ± SEM of specimens obtained from a minimum of 4 different animals. c Representative western blot showing expression of Ang1, Ang2, Tie 2, VEGF-A and vinculin. d-f VEGF-A, Ang1 and Ang2 expression quantified by densitometry with normalization to vinculin. Each column is representative of the mean ± SEM of values obtained from a minimum of 4 different animals. g Ratio of the normalized expression of Ang1 to Ang2 with each column representative of the mean ± SEM of matched values obtained from a minimum of 4 different animals. h Tie2 expression quantified by densitometry with normalization to vinculin, each column represents the mean ± SEM of a minimum of 4 different animals. i Representative western blot showing expression of interleukin (IL)-6, IL-1β and vinculin. j and k IL-6 and IL-1β expression quantified by densitometry with normalization to vinculin. Columns are representative of the mean ± SEM of values obtained from a minimum of 3 different animals. * p < 0.05, ** p < 0.01, # p < 0.001, ## p < 0.0001