Fig. 5

Effect of a miR-451 inhibitor on vascular vessel density measured by von Willebrand Factor (vWF) immunostaining. Immunostaining showing vascular staining of pulmonary vessels by vWF, a marker for blood vessels. In room air (RA), there are more vessels with intact vascular wall (a) as compared to the BPD group (c), where the vessels are disrupted and do not have smooth walls (see inset). After administration of miR-451 inh (d), the pulmonary phenotype of the alveolar sacs are restored, there are increased number of intact blood vessels, and the vascular wall regain their shape (inset). The inhibitor did not have any deleterious effect in the RA-treated group (b). Side panel shows quantification of blood vessels in different groups after counting each region of the lung in high power field. HPF: High power field; * p < 0.05; # p < 0.001; Scale Bar: 100 μm; inset scale bar: 10 μm. Blue: 4′,6-diamidino-2-phenylindole (DAPI); Red: vWF