Fig. 3

Expression of miR-223 and miR-374a correlate to eosinophil levels in non-allergic asthmatics. Expression of two circulating miRNAs were observed to change based on eosinophil numbers. Individuals with non-allergic asthma were divided based on circulating eosinophil cell level- where High Eos are > 0.4 × 10^9 cells/L and healthy subjects and Low Eos individuals had < 0.1 × 10^9 cells/L (a and c) or eosinophil level and ICS usage (b and d). (e) A flow chart to describe miRNA expression for each group is shown. ↑ indicates increased expression compared to healthy, ↑ indicates increased expression compared to healthy, ↓ indicates decreased expression compared to healthy and – indicated no change from healthy. Each dot is equivalent to one subject. Healthy = 24, NAA High Eos = 23, NAA Low Eos = 22, NAA High Eos + ICS = 13, NAA High Eos-ICS = 10, NAA Low Eos + ICS = 16, NAA Low Eos-ICS = 6. Expression is shown as 2^-dCt. Kruskal-Wallis followed by Dunns post-test was performed. p < 0.05 = *; p < 0.01 = **; p < 0.001 = ***; p < 0.0001 = ****