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Fig. 5 | Respiratory Research

Fig. 5

From: Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells

Fig. 5

EVs induce TGF signaling in A549 epithelial cells. a Immunoblotting of phosphorylated SMAD2 expression over time in epithelial cells after incubation with 30 μg/ml of EVs and 10 ng/ml of TGFβ-1. Representative images are shown from three independent experiments. b Immunoblot analysis of phosphorylated SMAD2 in A549 cells exposed to EVs after pre-treatment with a blocking antibody against TGFβ-1. Representative images are shown from two independent experiments. c TGFβ-1 levels in the culture supernatants from epithelial cells that were exposed to EVs, as detected by ELISA

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