Fig. 3
From: Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells
EVs induce an EMT-like phenotype in lung epithelial cells. a Immunoblotting analysis of NCAD, ECAD, and Slug-Snail protein in A549 cells after 24-h incubation with EVs (30 μg/ml). Representative images are shown from three independent experiments. b Immunofluorescence imaging of A549 cells to determine the cellular distribution of NCAD (green) and DAPI (red). Images were acquired at 20× magnification after 48 h of EV treatment (30 μg/ml). c Gelatin zymography analysis of culture supernatant of EV-treated A549 cells to determine the matrix metalloproteinase activity of MMP-9 and MMP-2. d Boyden chamber reverse trans-well migration assay of epithelial cells towards EVs (30 μg/ml). Representative images are shown from three independent experiments