Heme metabolism genes Downregulated in COPD Cachexia

Respiratory Research

Table 3 Genes significantly differentially expressed between cachectic¹ and non-cachectic COPD

Gene	Name	COPDGene		ECLIPSE³
Gene	Name	Log FC	FDR-value²	Log FC	FDR-value²
ALAS2	5′-Aminolevulinate Synthase ²	− 0.42	0.015	−0.98	0.00010
ANK1	Ankyrin 1, Erythrocytic	−0.23	0.036	−0.80	0.0060
ASCC2	Activating Signal Cointegrator 1 Complex Subunit ²	−0.21	0.0074	−0.53	0.012
BACH2	BTB Domain and CNC Homolog ²	−0.17	0.0074	–	–
CDC34	Cell Division Cycle 34	−0.22	0.011	−0.58	0.0066
CSF2RB	Colony Stimulating Factor 2 Receptor Beta Common Subunit	−0.12	0.036	–	–
ENSG00000231393	Novel Transcript	−0.45	0.036	–	–
ENSG00000236283	Novel Transcript	−0.68	0.046	–	–
GUCD1	Guanylyl Cyclase Domain Containing ¹	−0.17	0.0088	−0.36	0.0060
IGHG1	Immunoglobulin Heavy Constant Gamma ¹	0.59	0.0074	–	–
MIR186	MicroRNA 186	−0.48	0.0074	–	–
PLEK2	Pleckstrin ²	−0.33	0.044	− 1.04	0.0027
PPP2R5B	Protein Phosphatase 2 Regulatory Subunit B’Beta	−0.26	0.012	−0.30	0.012
RILP	Rab Interacting Lysosomal Protein	−0.24	0.012	−0.51	0.012
RNF149	Ring Finger Protein 149	−0.087	0.0078	–	–
SLC25A39	Solute Carrier Family 25 Member 39	−0.22	0.0074	−0.57	0.0053
SMIM24	Small Integral Membrane Protein ²	−0.66	0.0074	−0.71	0.025
SPTB	Spectrin Beta, Erythrocytic	−0.33	0.0074	−0.77	0.0060
TICAM1	Toll Like Receptor Adaptor Molecule ¹	0.48	0.016	–	–
TNS1	Tensin ¹	−0.31	0.018	−1.07	0.0010
TRIM58	Tripartite Motif Containing 58	−0.34	0.0074	−0.90	0.0036
TRPM2	Transient Receptor Potential Cation Channel Subfamily M Member ²	0.30	0.016	–	–
UBXN6	UBX Domain Protein 6	−0.21	0.032	−0.55	0.012

FC Fold Change
¹In COPDGene, cachexia was defined at visit two, approximately five years from baseline, as either unintentional weight loss greater than 5% in the past year or low BMI in addition to one of three criteria: low 6MWD, anemia or low FFMI. Unintentional weight loss was calculated at visit two using self-reported unintentional weight loss in the past year. Low 6MWD and anemia were classified using visit two data. FFMI was derived from pectoralis muscle area on baseline chest computed tomography scans [18]. In ECLIPSE, cachexia was defined as weight loss greater than 5 % in the past 12 months or low BMI and at least three of the five criteria: low six-minute walking distance, anorexia, abnormal biochemistry (anemia or high CRP), fatigue, and low FFMI
²In COPDGene, FDR was calculated based on 65,988 transcripts. In ECLIPSE, FDR was calculated based on N = 14 significantly differently expressed probes present in COPDGene
³Missing values were not significantly replicated in ECLIPSE

ISSN: 1465-993X