Fig. 5
From: TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung
SMC development and its correlation with TBX2 expression. (a, c) Double immunofluorescence analysis of expression of TBX2 with the SMC protein TAGLN on frontal lung sections of control mice (a), of Tbx2cre/+;HprtTBX2/y lungs at E12.5, and of 8-day cultures of E12.5 Tbx2+/+;HprtTBX2/+ and Tbx2cre/+;HprtTBX2/y lung explants (c). (b) Quantification of TBX2 expression in pulmonary SMCs marked by TAGLN or ACTA2 of control embryos at different embryonic stages. Average values: TAGLN+: 93% ± 6% (E11.5); 99% ± 5% (E12.5); 81% ± 23% (E14.5); 63% ± 3% (E16.5); ACTA2+: 98 ± 1% (E11.5); 87% ± 14% (E12.5); 87 ± 12% (E14.5); 39% ± 3% (E16.5). Complete data set is provided in Table S1. Technical artefacts produce values above 100%. (d) Immunofluorescence of TAGLN (red) on control and Tbx2cre/fl;R26mTmG/+ frontal lung sections at different developmental stages. Antigens are color-coded. Stages are as indicated. Nuclei were counter stained with DAPI. Insets of overview images are magnified in the row below. ca: caudal; cr: cranial; d: dorsal; f: foregut; l: left; lb.: lung bud; r: right; v: ventral