Skip to main content
Fig. 4 | Respiratory Research

Fig. 4

From: TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung

Fig. 4

Tbx2-overexpression does not alter the fate of TBX2+ cells in the developing lung. (a) Morphology and GFP/RFP epifluorescence of lung explants of E12.5 Tbx2cre/+;R26mTmG/+ (control), Tbx2cre/+;HprtTBX2/+ (female) and Tbx2cre/+;HprtTBX2/y (male) embryos at day 0 and day 6 of culture. (b) Double immunofluorescence analysis of YFP (indicating TBX2 expression from the Hprt allele) and cell-type specific marker proteins (TAGLN, ACTA2 for SMCs; EMCN for the endothelium; CDH1 for the epithelium; S100A4 for different types of fibroblasts and POSTN for the ECM) on sections of Tbx2cre/+;HprtTBX2/y lung rudiments after 8 days of culture. Antigens are color-coded. Selected regions of overview images are magnified in the row below. (c) Immunofluorescent analysis of TAGLN expression on frontal sections of Tbx2cre/+;R26mTmG/+ (control), Tbx2cre/+;HprtTBX2/+ and Tbx2cre/+;HprtTBX2/y lung cultures. Genotypes are as indicated. Insets or selected regions in overview images are magnified in the row below. Nuclei were counterstained with DAPI

Back to article page