Fig. 3
From: TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung
Tbx2-deficiency does not alter the fate of TBX2+ cells in the developing lung. (a) Double immunofluorescence analysis of expression of the lineage marker GFP with SMC proteins (ACTA2, TAGLN), and with markers of the endothelium (EMCN, KDR), the visceral pleura (ALDH1A2, WT1), different types of fibroblasts (S100A4, PDGFRA, PDGFRB) and the ECM (POSTN) on frontal lung sections of Tbx2-deficient (Tbx2cre/fl;R26mTmG/+) embryos at representative stages. (b) Quantification of the TBX2 lineage contribution reflected by GFP signal to the mesenchyme of the whole lung (E10.5) and the right lung lobe (E14.5 and E16.5) of Tbx2-deficient lungs. Average values: E10.5: 81 ± 11%; E14.5: 101% ± 2%; E16.5: 100% ± 1%. (c) Quantification of GFP signal in specific cell types (E14.5 and E16.5) upon Tbx2 deletion. Average values: ACTA2+: 104% ± 6%; TAGLN+: 82% ± 27%; EMCN+: 94% ± 25%; ALDH1A2+: 60% ± 12%; POSTN+: 86% ± 8%; PDGFRA+: 87 ± 11%; PDGFRB+: 85% ± 31%. Complete data set is provided in Table S1. Values above 100 result from technical artefacts. Antigens are color-coded, stages are as indicated. Nuclei were counterstained with DAPI. Selected regions of overview images are magnified in the row below. ca: caudal; cr: cranial; l: left; r: right.