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Fig. 1 | Respiratory Research

Fig. 1

From: TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung

Fig. 1

Tbx2/TBX2 is predominantly expressed in mesenchymal progenitors in the developing lung. (a, b, c, d) In situ hybridization analysis of Tbx2 expression (a, c), and double immunofluorescence analysis of expression of TBX2 with the epithelial marker CDH1 (b, d) on transversal (a, b) and frontal (c, d) sections of the lung at different stages of wildtype mouse embryos. (e) Double immunofluorescence analysis of expression of TBX2 and of marker proteins for SMCs (TAGLN, ACTA2), the endothelium (EMCN), the mesothelium of the visceral pleura (ALDH1A) and different types of fibroblasts and ECM (POSTN, S100A4, PDGFRA, PDGFRB) on frontal sections of E14.5 wildtype lungs. (f) Quantification of mesenchymal TBX2 expression in the whole lung (E10.5 and E12.5) and in the right lung lobe (E14.5 and E16.5). Average values: E10.5: 83% ± 18%; E12.5: 95% ± 8%; E14.5: 101% ± 3%, E16.5: 98% ± 2%. (g) Quantification of cell-type specific TBX2 expression at E14.5. Average values: ACTA2+: 87% ± 12%; TAGLN+: 81% ± 23%; EMCN+: 43% ± 10%; ALDH1A2+: 59% ± 9%; POSTN+: 89% ± 25%; PDGFRA+: 91% ± 10%; PDGFRB+: 73% ± 21%. Complete data set is provided in Table S1. Values above 100% result from technical artefacts. Stages, probes and antigens are as indicated. Nuclei were counter stained with DAPI in immunofluorescence stainings. Insets of overview images are magnified in the row below. ca: caudal; cr: cranial; d: dorsal; f: foregut; l: left; lb.: lung bud; r: right; v: ventral. Black arrow: indicates a blood auto-fluorescent cell; white arrow: TBX2 negative mesothelial cell; white arrowhead: TBX2+ endothelial cell; black arrowhead: TBX2+ mesothelial cell

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