Table 1 Primers for qRT-PCR analysis are listed. “CEBPA” primers were used to measure human fibroblast endogenous gene expression, and “Cebpa” primers used to detect the expression of pScalps_Puro_mCebpa plasmid
From: Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression
gene names | Sequences |
|---|---|
GAPDH-F | AATGAAGGGGTCATTGATGG |
GAPDH-R | AAGGTGAAGGTCGGAGTCAA |
CEBPA-F | GCAAACTCACCGCTCCAATG |
CEBPA-R | GGAAGGAGGCAGGAAACCTC |
ACTA2-F | GTGTTGCCCCTGAAGAGCAT |
ACTA2-R | GCTGGGACATTGAAAGTCTCA |
COL1Α1-F | GAGGGCCAAGACGAAGACATC |
COL1Α1-R | CAGATCACGTCATCGCACAAC |
FN1-F | AGGAAGCCGAGGTTTTAACTG |
FN1-R | AGGACGCTCATAAGTGTCACC |
CTGF-F | CAGCATGGACGTTCGTCTG |
CTGF-R | AACCACGGTTTGGTCCTTGG |
PLIN2-F | ATGGCAGAGAACGGTGTGAAG |
PLIN2-R | CAACTGCAATTTGCGGCTC |
PPARA-F | GCTTTCTGGGTGGACTCAAGT |
PPARA-R | GAGGGCAATCCGTCTTCATCC |
PPARG1-F | GGGATCAGCTCCGTGGATCT |
PPARG1-R | TGCACTTTGGTACTCTTGAAGTT |
PPARGC1A-F | GCTTTCTGGGTGGACTCAAGT |
PPARGC1A-R | GAGGGCAATCCGTCTTCATCC |
Cebpa-f | CAAGAACAGCAACGAGTACCG |
Cebpa-r | GTCACTGGTCAACTCCAGCAC |