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Fig. 5 | Respiratory Research

Fig. 5

From: Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression

Fig. 5

CEBPA expression can be restored by CRISPR activation. a) Schematic illustration of the process of CRISPR gene activation (reproduced image is from Horizon Discovery and used with their permission) b) qRT-PCR analysis of CEBPA expression in the dCAS9 expressing IPF fibroblasts with non-targeting gRNA or CEBPA gRNA or CEBPA gRNA&CEBPA siRNA together for 3 days. c-d) Western blot analysis and quantification of Fibronectin and αSMA expression in the dCAS9 expressing IPF fibroblasts with non-targeting gRNA or CEBPA gRNA or CEBPA gRNA&CEBPA siRNA together for 3 days. e-i) qRT-PCR analysis showing ACTA2, COL1A1, FN1, CTGF and PLIN2 transcript levels in the dCAS9 expressing IPF fibroblasts with non-targeting gRNA or CEBPA gRNA or CEBPA gRNA&CEBPA siRNA together for 3 days. Data are expressed as mean ± SD (*p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001)

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