Fig. 1

Saponin-based human DNA depletion effectively removed human DNA without changing bacterial community structure. a Before human DNA depletion, 1% of Nanopore reads were of microbial origin; following human DNA depletion, 48% of Nanopore reads were of Microbial origin. b There was no significant difference in alpha diversity of bacterial communities between depleted and undepleted samples assessed by 16S rRNA gene sequencing. c Non-metric multidimensional scaling (NMDS) plot of the Bray-Curtis dissimilarity index between depleted and undepleted samples based on 16S rRNA gene sequencing. Depleted samples were compositionally similar to undepleted samples (PERMANOVA, p-value = 0.17)