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Table 3 Comparison of EV Characterizing and Analyzing Techniques

From: Isolation and characterization of extracellular vesicles from Broncho-alveolar lavage fluid: a review and comparison of different methods

Technique DLS NTA nanoFACS
Feature Techniques based on the Brownian motion of and light scattering from the particle Techniques based on the dual forward scatter and single forward scatter
Measure the diffusion coefficient and derive the size from that diffusion coefficient. So both are influenced by shape in the same manner Enhanced Forward Scatter (eFSC) technology
Ensemble measurement provides an intensity-based distribution Particle-by-particle measurement provides a number-based distribution Expression of certain EV surface epitopes which can be stained by specific fluorescently labeled antibodies
Can provide the % by intensity of particles Can give the % by number of particles Valuable tool to study cell-type specific surface proteins or activation markers on exosomes.
To measure particles smaller than 10 nm or larger than micron Can often provide higher resolution, To gain insights about EV populations originating from a particular cell type