Table 3 Comparison of EV Characterizing and Analyzing Techniques
Technique | DLS | NTA | nanoFACS |
|---|---|---|---|
Feature | Techniques based on the Brownian motion of and light scattering from the particle | Techniques based on the dual forward scatter and single forward scatter | |
Measure the diffusion coefficient and derive the size from that diffusion coefficient. So both are influenced by shape in the same manner | Enhanced Forward Scatter (eFSC) technology | ||
Ensemble measurement provides an intensity-based distribution | Particle-by-particle measurement provides a number-based distribution | Expression of certain EV surface epitopes which can be stained by specific fluorescently labeled antibodies | |
Can provide the % by intensity of particles | Can give the % by number of particles | Valuable tool to study cell-type specific surface proteins or activation markers on exosomes. | |
To measure particles smaller than 10 nm or larger than micron | Can often provide higher resolution, | To gain insights about EV populations originating from a particular cell type | |