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Fig. 3 | Respiratory Research

Fig. 3

From: Down-regulation of long non-coding RNA SNHG14 protects against acute lung injury induced by lipopolysaccharide through microRNA-34c-3p-dependent inhibition of WISP1

Fig. 3

LncRNA SNHG14 and WISP1 bind to miR-34c-3p. A, the binding of miR-34c-3p to WISP1 was confirmed by dual-luciferase reporter assay, * p < 0.05 vs. the NC mimic group (cells transfected with NC mimic). B, interaction between miR-34c-3p and lncRNA SNHG14 or WISP1 determined by RIP assay. * p < 0.05 vs. the anti-IgG group (MH-S cells incubated with anti-IgG). C, mRNA expression of WISP1 after transfection with miR-34c-3p mimic or miR-34c-3p mimic and oe-SNHG14 determined by RT-qPCR, * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic), # p < 0.05 vs. the miR-34c-3p mimic + oe-NC group (MH-S cells co-transfected with miR-34c-3p mimic and oe-NC). D, Western blot analysis of WISP1 protein expression normalized to GAPDH after transfection with miR-34c-3p mimic or miR-34c-3p mimic and oe-SNHG14, * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic), # p < 0.05 vs. the miR-34c-3p mimic + oe-NC group (MH-S cells co-transfected with miR-34c-3p mimic and oe-NC). The statistical values were measurement data and expressed as mean ± standard deviation. Comparison was analyzed using unpaired t-test in panels A and B or using one-way ANOVA in panels C and D. The experiment was repeated 3 times independently

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