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Fig. 2 | Respiratory Research

Fig. 2

From: Down-regulation of long non-coding RNA SNHG14 protects against acute lung injury induced by lipopolysaccharide through microRNA-34c-3p-dependent inhibition of WISP1

Fig. 2

miR-34c-3p is down-regulated in ALI and its over-expression protects against LPS-induced inflammation. A, the binding relationship between miR-34c-3p and lncRNA SNHG14 determined by online software (upper panel) and dual-luciferase reporter assay (lower panel), * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic). B, miR-34c-3p expression in MH-S cells treated with LPS and mice with LPS-induced ALI determined by RT-qPCR, * p < 0.05 vs. the PBS + MH-S group (MH-S cells treated with PBS), n = 10. C, Pearson correlation analysis of miR-34c-3p and lncRNA SNHG14 expression. D, the effect of lncRNA SNHG14 silencing on the expression of miR-34c-3p detected by RT-qPCR, * p < 0.05 vs. the NC-ASO group (MH-S cells transfected with NC-ASO). E, the transfection efficiency of miR-34c-3p mimic detected by RT-qPCR, * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic). F, the levels of TNF-α and IL-6 in the MH-S cell supernatant were determined by ELISA. * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic). G, MH-S cell viability in response to miR-34c-3p mimic transfection assessed by CCK-8 assay, * p < 0.05 vs. the NC mimic group (MH-S cells transfected with NC mimic). The statistical values were measurement data and expressed as mean ± standard deviation. Comparison between two groups was analyzed using unpaired t-test. Statistical analysis in relation to time-based measurements within each group was realized using two-way ANOVA with non-repeated measure. The experiment was repeated 3 times independently

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