Skip to main content
Fig. 7 | Respiratory Research

Fig. 7

From: Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells

Fig. 7

TGF-β1 is secreted by human peripheral blood neutrophils and is increased in neutrophils from severe asthmatics -NHBE co-cultures. a Neutrophils from non-asthmatic and severe asthmatic individuals were stained with anti-human TGF-β1 antibodies. TGF-β1 was localized to the cytoplasm. Representative images are shown at 200x. b Active TGF-β1 was measured in culture supernatants by enzyme linked immunoscorbant assay. Culture supernatants were collected (from left to right) from untreated cultures of neutrophils (48 h, N = 5), NHBEs treated with neutrophil conditioned medium (48 h, N = 4) and NHBEs co-cultured with neutrophils (see methods, N = 4). Mean ± SE; P < 0.0001(****)

Back to article page