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Fig. 3 | Respiratory Research

Fig. 3

From: Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells

Fig. 3

Neutrophils from severe asthmatic patients induce changes in alpha smooth muscle actin mRNA in human bronchial epithelial cells consistent with EMT. qPCR analysis of Alpha smooth muscle actin mRNA expression of NHBEs following treatment with neutrophil conditioned medium (top panels) or when co-cultured with peripheral blood neutrophils (bottom panels). Neutrophils were obtained from non-asthmatic individuals (left panels) or severe asthmatic individuals (right panels). N = 4, Mean ± SE; P < 0.05(*), P < 0.01(**), P < 0.001(***), P < 0.0001(****)

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