Fig. 1

Evaluation of lung function measurement assessed by the NPFE maneuver in mice using orotracheal intubation and tracheostomy. a, b, Average expiratory flow-volume curves of intubated (“OTI”, in blue) and tracheostomized (“TRA”, in red) mice. n = 13 BALB/cJ mice, n = 10 C57BL/6 J mice. Lower and upper error bars represent standard deviations for intubation and tracheostomy, respectively. c-f, Forced vital capacity (“FVC”, c and e), forced expired volume over 0.1 s (“FEV0.1”, d and f) in intubated and tracheostomized BALB/cJ mice (c, e) and C57BL/6 J mice (d, f). Data represent individual mice and are analyzed by the Wilcoxon signed-rank test or paired t tests. * P < 0.05. g, i, Relationships between FVC measured in intubated and tracheostomized BALB/cJ (g) and C57BL/6 J (i) mice. h, j, Relationships between FEV0.1 measured in intubated and tracheostomized BALB/cJ (h) and C57BL/6 J (j) mice. k-n, Bland-Altman plots to compare two measurements techniques for FVC in BALB/cJ (k) and C57BL/6 J (m), FEV0.1 in BALB/cJ (l) and C57BL/6 J (n). The upper and lower limits of agreement (95% confidence interval) are shown by a gray dotted line