Skip to main content
Fig. 1 | Respiratory Research

Fig. 1

From: Wnt signaling regulates trans-differentiation of stem cell like type 2 alveolar epithelial cells to type 1 epithelial cells

Fig. 1

Gene expression changes during AT2-to-AT1 TD. Primary lung epithelial cells were isolated from lung tissue samples of lobectomy patients with normal lung function, then were cultured on collagen-coated plastic plates. mRNA was obtained on days 2, 3 and 6 of culture and real-time qPCR analysis was performed using Taqman® probes. GAPDH was used as a housekeeping gene. Affymetrix microarrays were also performed at each time points. a mRNA expression of AT1 marker AQP5 (n = 3/groups); b mRNA expression of AT2 marker SPC (n = 3/groups); c Summary of PANTHER analysis of Affymetrix microarray analysis. Numbers indicate the number of genes which expression is changed in day 6 of culture compared to day 3. d) Heat-map of Wnt signaling pathway-associated genes that are significantly (p < 0.01) changed during AT2-to-AT2 TD (n = 3). 73 genes associated with the Wnt pathway are changing significantly (p < 0.01) during the in vitro TD process. e Gene expression levels of Wnt ligands Wnt4, Wnt5a, Wnt7a and receptors Fzd1, Fzd2 and Fzd7 following in vitro TD using microarray (n = 3). Mann-Whitney test * p < 0.05, ** p < 0.01, *** p < 0.001

Back to article page