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Fig. 7 | Respiratory Research

Fig. 7

From: Interferon-γ enhances the antifibrotic effects of pirfenidone by attenuating IPF lung fibroblast activation and differentiation

Fig. 7

IFN-γ and PFD reverse the effect of TGF-β1 on MMP and TIMP gene expression. NHLFs [black bars, left y-axis] and IPF fibroblasts [gray bars, right y-axis] were treated with INF-γ [10–30 ng/ml], PFD [300–500 μg/ml] or a combination of IFN-γ/PFD in the presence of TGF − β1 [2.5 ng/ml] for 3 days. MMP3 (a), MMP9 (b), MMP1 (c), MMP7 (d), TIMP1 (e) and TIMP2 (f) gene expression was measured by qPCR. Data for the relative quantification of each target gene are plotted as 2-ΔΔCT (mean fold change) using the YWHAZ housekeeping gene as a control. Data are presented as the mean ± SEM of 3 independent experiments, with each experiment run in duplicate. **P < 0.05 vs. TGF-β1 and ## P < 0.05 vs. TGF-β1+ vehicle [DMSO]

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