Fig. 2
Emphysema-like changes in mice exposed to cigarette smoke. Mice wild-type (WT), CFTR-heterozygous (Het), and CFTR-knockout (KO) were exposed to filtered air (FA) or cigarette smoke (CS) for 10 months. (a) Representative micrographs of lung parenchyma stained with hematoxylin and eosin and (b) mean linear intercepts (LM) of wild-type (WT), CFTR-heterozygous (Het), and CFTR-knockout (KO) C57BL/6 J mice exposed to filtered air (FA) or cigarette smoke (CS) for 10 months. N = 4-9 mice per group. (c) Percentage of monocytes/macrophages in bronchoalveolar lavage fluid of mice exposed to cigarette smoke for 4 weeks. N = 8 from each group. Magnification: 400x. Scale bar = 50 μm. * p < .05, *** p < .001 between groups indicated by line; ## p < .01, ### p < .001 compared to FA mouse of same genotype. NS indicates there is no significant difference between FA and CS WT mice. Significance was determined via one-way ANOVA with Tukey’s post-hoc multiple comparison