Fig. 5

Effect of ST2 deficiency and cohousing on the gut microbiome of male mice. DNA was extracted from fecal pellets of male mice prior to exposure. Results of 16S rRNA sequencing of fecal DNA harvested from male WT and ST2^−/− same housed and cohoused mice. a) Simpson index; b) Taxa relative abundances at the phylum level; c) Top 10 genera taxa abundances relative to total gut Bacteria and Archaea reads. d) Lactobacillus genus determined by MaAsLin as significantly affected by housing (p = 0.0015, q = 0.196). In a, b, and d bars indicate mean ± SEM. In a and d, each symbol indicates one mouse. In c, each column indicates one mouse. # p < 0.05 vs WT mice, + p < 0.05 vs same-housed mice by Factorial ANOVA. Statistics were performed on arcsine-square root transformed data