Table 1 Real-time PCR templates and primers used for gene manipulation
Gene symbol | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
SNAIL | CCTCTCACTGGGTCTTCTGG | GGTCTTCTTCCGCTCCTCTC |
AQP5 | GCTGCCATCCTTTACTTCTACC | GGTCTTCTTCCGCTCTTCC |
FIBRONECTIN | CCAAGCAGGAGTCAAACGAG | TCTTCCATCTCACGCATCTG |
α-SMA | CCGACCGAATGCAGAAGGA | ACAGAGTATTTGCGCTCCGAA |
CDH1 | GGTCTCTCTCACCACCTCCA | CCTCGGACACTTCCACTCTC |
CDH2 | CGTGAAGGTTTGCCAGTGT | CAGCACAAGGATAAGCAGGA |
SP-C | CCTTCTTATCGTGGTGGTGGT | TCTCCGTGTGTTTCTGGCTCAT |
GAPDH | GACAACAGCCTCAAGATCATCAG | ATGGCATGGACTGTGGTCATGAG |