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Fig. 4 | Respiratory Research

Fig. 4

From: IL-22Ra1 is induced during influenza infection by direct and indirect TLR3 induction of STAT1

Fig. 4

Type I interferon signaling is responsible for IL-22Ra1 induction following H1N1 infection. A549s were pretreated with 5 μg/ml of anti-IFNα receptor 2 antibody, αIFNAR2, for 30 min. Cells were then treated with Poly (I:C) (50 μg/ml) for 12 h. Cell were then collected in trizol and RNA isolated for RT-qPCR analysis of IL-22Ra1. αIFNAR2 inhibited induction of IL-22Ra1 confirming that Type I interferon signaling was responsible for induction of IL-22Ra1 following H1N1 infection (one-way ANOVA, p = 0.0055). Representative of two experiments

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