Fig. 1
From: IL-22Ra1 is induced during influenza infection by direct and indirect TLR3 induction of STAT1
TLR3 mediated induction of IL-22Ra1 by Poly (I:C) and H1N1 in vitro and in vivo. a Mice were treated oropharyngeally with Poly (I:C) (50μg/ml), H1N1 (100 pfu) or LPS (2 μg/ml) for 24 h respectively then bronchial brushings were performed. RNA was then isolated for RT-qPCR analysis of Il-22ra1. Both Poly (I:C) and H1N1 significantly induced Il-22ra1. (one way ANOVA p < 0.0001). Induction was also confirmed by immunofluorescence of IL-22Ra1 in H1N1 infected (MOI 50) A549 cells. b Untreated control vs. H1N1 24 h.p.i (both at 20x magnification). c Quantification of immunofluorescent IL-22Ra1 induction [t-test, p = 0.0005,n = 6 (20 pictures per condition)]. Representative of two experiments. Cells were treated with Poly (I:C) (50μg/ml) or H1N1 (MOI 50) respectively then RNA was then isolated for RT-qPCR analysis of IL-22Ra1 in d.) A549s (t-test p = 0.0167, n = 6) and e.) BEAS-2Bs, a primary airway cell line (t-test p < 0.0001,n = 9)