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Table 1 Protein structure prediction results for the two GSTCD isoforms using three models I-TASSER, SWISS- MODEL and Phyre2. Methyltransferase (MTF) enzymes are frequently found in the top 3 structural templates listed for each server and the top 50 template results in SWISS-MODEL were entirely methyltransferase proteins, with specific focus on rRNA methyltransferase function. Alternatively, I-TASSER identified homology in this region with transport receptors, specifically importin β subunit 1, involved in transporting proteins into the nucleus [25]. Other top hits included: exodeoxyribonuclease (catalyses degradation of double stranded DNA), Ras guanyl-releasing protein 1 (nucleotide exchange factor specifically activating Ras, activates the extracellular signal-regulated kinases/mitogen-activated protein kinase (ERK/MAPK) cascade) and nicotinamide adenine dinucleotide binding (NADB)-Rossmann fold superfamily protein (structural motif found in proteins that bind nucleotides)

From: Defining a role for lung function associated gene GSTCD in cell homeostasis

Protein Structure Results I-TASSER SWISS-MODEL Phyre2
Variant 1 Variant 2 Variant 1 Variant 2 Variant 1 Variant 2
Predicted Secondary Structure Primarily α-helices with intermittent coil structures.
β-strands only present at termini.
N/A Primarily intermittent α-helices (45%).
β-strands only present at termini (12%).
Two predicted transmembrane regions (30-40aa and 281-299aa).
Primarily intermittent α-helices (55%).
β-strands only present at termini (10%).
Two predicted transmembrane regions (29-38aa and 194-212aa).
Regions of Primary Sequence Homology Primarily across the MTF domain to C-terminus.
Partial matches across whole protein.
Only across the MTF domain to C-terminus Only across the MTF domain
Intrinsic disorder N/A Low disorder across C-terminus High disorder across C-terminus Two theoretical central regions of disorder (27%):• 204-270aa• 325-386aa Two theoretical central regions of disorder (26%):• 118-184aa• 257-299aa
Top 3 Structural Templates Putative MTF
Importin β subunit
Exodeoxyribonuclease
Ras guanyl-releasing protein 1
MTF
Protein RdmB MTF
Protein RdmB MTF
Protein RdmB MTF
Putative rRNA methylase
Putative rRNA methylase
rRNA small subunit MTF
rRNA small subunit MTF
Transferase
S-adenosyl-L-methionine-dependent MTF
Transferase
NADB-Rossmann fold superfamily protein
S-adenosyl-L-methionine-dependent MTF