Fig. 3
From: Defining a role for lung function associated gene GSTCD in cell homeostasis
GSTCD siRNA knock-down in hBECs results in reduced total cell number while not influencing proliferation. GSTCD siRNA knock-down in hBECs does not affect proliferation rate (Click-iT® EdU) but does result in reduced total cell number over 72 h (CyQuant) in cell culture. Graphs show median and interquartile range data analysed using Kruskal-Wallis test. a. The Click-iT® EdU assay data is normalised to Untransfected controls (100%) bar. No change in proliferation rate was seen in GSTCD knock-down samples (siRNA B and C) relative to untransfected or scrambled controls at any time-point. b. The CyQuant assay shows a noticeably reduced total cell number in both GSTCD knock-down samples, with an increasing effect across the time-course and significant reduction evident at 72 h compared to scrambled for siRNA C (p = 0.02).