Fig. 2
From: Defining a role for lung function associated gene GSTCD in cell homeostasis
GSTCD can effectively be targeted in vitro leading to a reduction in mRNA and protein levels. a. Shows RNA expression using taqman normalised to the untransfected condition. Both SiRNA B and C knocked down the GSTCD RNA to a similar extent. b. Western blots to show the GSTCD protein levels (71 kDa) are reduced in hBEC lysates following siRNA knock-down. GSTCD protein levels were noticeably reduced relative to untransfected and scrambled controls for each siRNA in all samples (n = 3). c. Semi-quantification of GSTCD protein levels by densitometry. Proportional changes are shown with results normalised against untransfected control. GSTCD protein expression is significantly reduced for siRNA B (p < 0.0036) and siRNA C (p < 0.036) using Kruskal Wallis test showing median and interquartile range. N = 3 independent experiments