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Fig. 1 | Respiratory Research

Fig. 1

From: Mass spectrometry imaging as a tool for evaluating the pulmonary distribution of exogenous surfactant in premature lambs

Fig. 1

Workflow of MSI analysis. The right lungs of lambs were cut into four pieces of approximately 3 cm (a) and the lower face of the third piece was cryo-sectioned and thaw-mounted onto a conductive glass slide. Samples were then spray-coated with an appropriate MALDI matrix (b). Samples were then analyzed with MALDI-TOF/TOF instrumentation, acquiring spectra at defined spatial intervals (c). Lastly, data were elaborated to create 2D ion intensity maps of surfactant Protein B and C analogs and tissue slices were stained with Hematoxylin and Eosin (d)

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Respiratory Research

ISSN: 1465-993X

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