Fig. 3
From: Anti-fibrotic effects of tannic acid through regulation of a sustained TGF-beta receptor signaling
Inhibition of bleomycin-induced pulmonary fibrosis by tannic acid. Tannic acid (TA) treatment (10 mg/kg, i.p., daily) started 7 days after intratracheal administration of bleomycin, and mice were sacrificed on day-21. a, representative images of H&E staining of paraffin-embedded lung sections. b-d, Histologic assessment of h&e-stained sections for a degree of fibrosis: b, Ascroft score; c, lung tissue surface area; d, histologic score. e, hydroxyproline assay of lung extracts for a content of collagen. f-h, Western blot analysis of lung extracts for a content of Laemmli-buffer-soluble collagen-1, phosphorylated Smad2 (P-Smad2) and β-actin (f), and ECL intensity of Collagen-1 (g) and P-Smad2 (h) blots; mean ± SD