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Fig. 2 | Respiratory Research

Fig. 2

From: Anti-fibrotic effects of tannic acid through regulation of a sustained TGF-beta receptor signaling

Fig. 2

Tannic acid inhibits sustained Smad2 phosphorylation and Smad-dependent gene transcription without affecting initial Smad2 phosphorylation in response to TGF-β. a, Pretreatment of HLF with tannic acid (TA) does not affect initial Smad2 phosphorylation in response to TGF-β (1 ng/ml). Shown are the representative results for P-Smad2 and Smad2 Western blotting from one of two independent experiments. b, Pretreatment with tannic acid inhibits TGF-β-induced Smad-dependent gene transcription, as assessed using SBE-luciferase reporter (shown are the representative results from one of two independent experiments performed in triplicates). c-f, Tannic acid inhibits the sustained phosphorylation of Smad2 in response to TGF-β. c, Representative P-Smad2 and Smad2 Western blot images; d-f, Densitometry of P-Smad2 (d), Smad2 (e), and normalized P-Smad2 / Smad2 (f) images. Shown are the means ± SD from at least three independent experiments

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