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Table 2 Gene function annotation enrichment analysis of the top up-regulated and down-regulated genes during differentiation on ALI culturea

From: Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity

Term

Gene numberb

Percentage of genes in the list (%)

p valuec

p value after Benjamini-Hochberg correction

Top up-regulated

 Cilium

68

4.7

2.3 × 10−28

1.1 × 10−25

 Cell projection

127

8.9

1.9 × 10−23

4.6 × 10−21

 Immunity

101

7.0

3.4 × 10−23

5.3 × 10− 21

Top down-regulated

 Ribosomal protein

92

6.3

5.1 × 10−57

2.5 × 10−54

 Ribonucleoprotein

102

6.9

4.0 × 10−45

9.6 × 10−43

 Acetylation

380

25.9

2.6 × 10−26

4.1 × 10−24

  1. aFunction term annotation enrichment analysis was carried out using DAVID analysis (https://david.ncifcrf.gov/tools.jsp). The fold-changes of RNAseq data between hSABCi NS1.1 cells on ALI-day 28 was compared to basal cells before ALI culture. The top 1500 up-regulated genes and top 1500 down-regulated genes were used for analysis. The top 3 enriched functional terms in the results were shown. See Methods for detail
  2. bNumbers of genes with the function described in “Term”
  3. cp value is from a modified Fisher exact test, indicating whether the gene list is enriched with an annotated function or not