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Table 1 Expression of airway epithelial lineage markers in hSABCi-NS1.1 cells during differentiation on ALI culturea

From: Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity

Cell lineagesb Gene symbol Gene name Expression level (FPKM)c
Basal ALI-d28
Basal cells KRT5 Keratin 5 130.9 121.2
  KRT14 Keratin 14 38.9 0.1
  TP63 Tumor protein p63 38.2 12.4
  KRT15 Keratin 15 1009.4 173.6
Ciliated cells FOXJ1 Forkhead box J1 0.0 42.4
  DNAI1 Dynein axonemal intermediate chain 1 0.0 21.3
  SPAG6 Sperm associated antigen 6 0.0 7.6
  TEKT1 Tektin 1 0.1 40.5
Secretory cells SCGB1A1 Secretoglobin family 1A member 1 35.6 6695.5
  SCGB3A1 Secretoglobin family 3A member 1 19.0 451.6
  MUC5B Mucin 5B, Oligomeric mucus/gel-forming 2.0 22.3
  LYZ Lysozyme 1.3 8.8
Alveolar cells SFTPA1 Surfactant protein A1 0.0 0.1
  SFTPA2 Surfactant protein A2 0.0 1.0
  SFTPB Surfactant protein B 0.0 3.6
  SFTPC Surfactant protein C 0.0 0.0
  SFTPD Surfactant protein D 0.1 0.5
Neuroendocrine cells CHGA Chromogranin A 0.0 0.0
CHGB Chromogranin B 0.8 1.2
  CALCA Calcitonin-related polypeptide alpha 0.1 0.0
  SCG5 Secretogranin V 0.1 6.3
Ionocytes FOXI1 Forkhead Box I1 0.0 0.1
  ATP6V1B1 ATPase H+ transporting V1 subunit B1 1.2 6.0
  CFTR Cystic fibrosis transmembrane conductance regulator 0.0 11.4
  1. aExpression level in hSABCi NS1.1 cells on ALI-day 28 compared to the basal cells prior to ALI culture were assessed by RNAseq
  2. bLineage markers were selected from the literature [12, 17, 24,25,26,27]
  3. cFPKM - fragments per kilobase of transcript/million mapped reads