Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity

Table 1 Expression of airway epithelial lineage markers in hSABCi-NS1.1 cells during differentiation on ALI culture^a

Cell lineages^b	Gene symbol	Gene name	Expression level (FPKM)^c
Cell lineages^b	Gene symbol	Gene name	Basal	ALI-d28
Basal cells	KRT5	Keratin 5	130.9	121.2
	KRT14	Keratin 14	38.9	0.1
	TP63	Tumor protein p63	38.2	12.4
	KRT15	Keratin 15	1009.4	173.6
Ciliated cells	FOXJ1	Forkhead box J1	0.0	42.4
	DNAI1	Dynein axonemal intermediate chain 1	0.0	21.3
	SPAG6	Sperm associated antigen 6	0.0	7.6
	TEKT1	Tektin 1	0.1	40.5
Secretory cells	SCGB1A1	Secretoglobin family 1A member 1	35.6	6695.5
	SCGB3A1	Secretoglobin family 3A member 1	19.0	451.6
	MUC5B	Mucin 5B, Oligomeric mucus/gel-forming	2.0	22.3
	LYZ	Lysozyme	1.3	8.8
Alveolar cells	SFTPA1	Surfactant protein A1	0.0	0.1
	SFTPA2	Surfactant protein A2	0.0	1.0
	SFTPB	Surfactant protein B	0.0	3.6
	SFTPC	Surfactant protein C	0.0	0.0
	SFTPD	Surfactant protein D	0.1	0.5
Neuroendocrine cells	CHGA	Chromogranin A	0.0	0.0
Neuroendocrine cells	CHGB	Chromogranin B	0.8	1.2
	CALCA	Calcitonin-related polypeptide alpha	0.1	0.0
	SCG5	Secretogranin V	0.1	6.3
Ionocytes	FOXI1	Forkhead Box I1	0.0	0.1
	ATP6V1B1	ATPase H+ transporting V1 subunit B1	1.2	6.0
	CFTR	Cystic fibrosis transmembrane conductance regulator	0.0	11.4

^aExpression level in hSABCi NS1.1 cells on ALI-day 28 compared to the basal cells prior to ALI culture were assessed by RNAseq
^bLineage markers were selected from the literature [12, 17, 24,25,26,27]
^cFPKM - fragments per kilobase of transcript/million mapped reads

ISSN: 1465-993X