Fig. 5

Differentiation capacity of immortalized hSABCi-NS1.1 cells on ALI culture. Passage 47 to passage 56 of hSABCi-NS1.1 cells were assessed on ALI day 14 to 35 for airway epithelial cell lineage markers. a Ciliated and club cells of passage 49 cells, ALI-day 21. Red-ARL13B; Green-SCGB1A1. Inset, higher magnification, showing cilia and SCGB1A1⁺ granules. Bar = 20 μm. b Mucous cells in passage 49 cells, ALI-day 16. Red-MUC5AC; green-MUC5B; yellow-co-localization (arrowheads). Bar = 20 μm. c Surfactant protein expressing cells, passage 49, ALI-day 35. Left, red-SFTPA; right, red-SFTPB. In both fields, nuclei were stained with DAPI (blue). d Ionocyte, passage 47, ALI-day 28. Red-FOXI1. Nuclei were stained with DAPI (blue). e Neuroendocrine cells, passage 49 cells, ALI-day 35. Red-CHGA. Nuclei were stained with DAPI (blue). Bar = 10 μm