Fig. 4

Cell junction formation of the immortalized hSABCi-NS1.1 cells on air-liquid interface (ALI) culture. a Trans-epithelial electrical resistance (TEER). Each color represents one independent experiment (n = 7 in total, passage 47 to 56 cells). Each dot represents average TEER of 3 ALI wells between day 0 and day 35 of ALI culture. b Junction protein staining of hSABCi-NS1.1 on ALI culture. Shown is tight junction protein 1 (TJP1) staining (green) of passage 47 cells on ALI-day 35. Bar = 20 μm. This composite image was created from a stack of individual images acquired at different depths in the monolayer (z-stack covering 40 μm). As a result, the merged image includes cell junction staining at the apical surface overlaid on the image of the epithelial cell nuclei. The difference in depth of the two structures accounts for difference in alignment of nuclei and apical tight junctions