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Fig. 3 | Respiratory Research

Fig. 3

From: Characterization of an immortalized human small airway basal stem/progenitor cell line with airway region-specific differentiation capacity

Fig. 3

Maintenance of basal cell features in the immortalized hSABCi-NS1.1 cells. a Basal cell markers staining at passage 6. Left, TP63 (green); middle, KRT5 (red); right, merged image. Bar = 50 μm. b Basal cell marker staining at passage 49. Left, TP63 (red); middle, KRT5 (green); right, merged image. Bar = 20 μm. Note that some nuclei appear negative for TP63 because of image contrast. c Expression of TP63 mRNA. Gene expression was assessed by TaqMan PCR. Each circle is one passage of hSABCi-NS1.1 cells. Primary small airway basal cells from 3 healthy nonsmokers at passage 2 were used as controls (blue triangle on right)

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Respiratory Research

ISSN: 1465-993X

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