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Fig. 5 | Respiratory Research

Fig. 5

From: Systemic Immuno-metabolic alterations in chronic obstructive pulmonary disease (COPD)

Fig. 5

CS exposure alters cellular metabolism in mouse macrophage cells. % Change in OCR (a) in RAW 264.7 cells after exposure to CSC for 1 h in the presence of glucose (10 mM) and puyruvate (2 mM) was measured using the XF Extracellular Flux analyzer as described in the material and methods section. % Change in OCR (Palmitate-BSA, 200 μM) and ECAR (glucose 10 mM)) in RAW 264.7 cells after exposure to CSC for 1 h (b). GAPDH enzyme activity was measured by monitoring consumption of NAD at 340 nm in RAW 264.7 cellular lysates after exposure to CSC for 1 h as described in the materials and methods section (c). * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared to their respective control using the Student’s t-test

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