Fig. 5
C5 inhibition mitigates IL-4 production and percentage of TH2 cell in the mLN following HDM challenge. Mediastinal lymph nodes (mLN) were harvested from HDM sensitized and challenged mice in both control and anti-C5 groups. In one experiment mLN cells were stimulated with either saline (NaCl) or HDM for four days. In cell-free supernatants the cytokines IL-4, IL-5 and IL-13 (a-c) were measured. In a separated experiment mLN cells were incubated with PMA and ionomycin in the presence of Golgiplug, followed by intracellular staining of IL-4 (d) and intranuclear staining of GATA-3 (e). Data are depicted as means ± SEM (n = 6–8 per group). #P < 0.05, ##P < 0.01, ***P < 0.001 for comparison between NaCl and HDM within control or anti-C5 mice