Fig. 3

C5 inhibition reduces T_H2 cells but not ILC2 following HDM challenge. Flow cytometry analysis of group 2 innate lymphoid cells (ILC2) and T-helper 2 cells (T_H2) in lung tissue. All cells depicted here are pre-gated as single, viable and CD45⁺ cells. (A) Total ILCs were defined as CD45⁺Lin⁻CD3⁻CD49b⁻ CD90⁺ cells. Within the total ILC population ST2⁺ cells were regarded as ILC2 cells. (B) Total T-helper cells were defined as CD45⁺CD4⁺ cells. T_H2 cells within total T-helper cell population stain positive for the transcription factor GATA-3. All data are means ± SEM (n = 8 per group). #P < 0.05 ***P < 0.001 for comparison between NaCl and HDM within control or anti-C5 mice