Fig. 5

Cftr ^−/− B cells have increased MHC class II and increased IL-6 response to LPS

MHC class II expression was measured on B cells from lung mononuclear cells a isolated from uninfected Cftr^−/− and wild type controls with directly conjugated antibodies by flow cytometry. b A representative histogram of MHC class II expression on lung B cells from uninfected Cftr ^−/− mice (gray histogram) and wild type controls (white histogram). Isotype control staining is represented by dotted line. MHC class II expression was also measured on B cell splenocytes isolated from uninfected Cftr ^−/− mice and wild type controls (c). d IL-6 production from B cells isolated from the spleens of Cftr ^−/− (n = 7) and WT mice (n = 4) stimulated with media or lipopolysaccharide (LPS) for 48 h was determined. The concentration of IL-6 in supernatants was determined by ELISA. Box plots show the median values and 25th and 75th percentiles, and error bars show the 10th and 90th percentiles. *p ≤ 0.05, Cftr ^−/− mice versus WT.