Fig. 6
From: Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia
Conjugation of fatty acids to Azm in treated airway epithelia. a) Total ion chromatograms of representative samples of control (blue) and Azm treated VA10 cells (red). Large peaks appear at 2.99 and 3.66 min in the Azm treated cells. b) Fragmentation of these peaks (2.99 peak shown here) shows many similarities to the fragmentation pattern of Azm but with an m/z shift of 239.3 in some of the fragments. This corresponds precisely to condensation of palmitate to one of the side-groups of AZM while releasing H2O. A similar pattern, with a shift of m/z = 267.3 was seen in the fragmentation of the 3.66 peak. This corresponds to condensation of stearate to Azm. c) Chemical structure of palmitate - Azm conjugate