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Fig. 4 | Respiratory Research

Fig. 4

From: Azithromycin induces epidermal differentiation and multivesicular bodies in airway epithelia

Fig. 4

Increased vesicle formation and lipid retention in Azm treated airway epithelial monolayer. a. Transmission electron microscope images showing that airway epithelia grown in monolayer (VA10 cells) treated with Azm have substantially more vesicle formation than the controls. Many of these vesicles were identified phenotypically as multivesicular bodies (MVB). Top scale bars are 5.0 μm and bottom scale bars are 1.0 μm. b. Increased lipid retention in Azm-treated VA10 cells seeded with HCS LipidTOX reagent. Representative images from 3 biological replicates. Top scale bar is 40 μm and bottom scale bars are 20 μm. c. Quantification of LipidTOX retention from confocal microscopy images in Fig. 4b. The mean pixel intensity of different wells from 3 different experiments was calculated using ImageJ (P < 0.01)

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