Fig. 4

IPF derived fibroblasts have low expression of HIF-3α by hypermethylation. Representative western blots of HIF3α (a) after 48 h of exposure to hypoxia (1% O2). β-actin was used as a loading control. Each bar represents the mean ± SD of the two fibroblast lines (b). Representative immunocytochemistry of HIF3α in normal fibroblast in normoxia and after 48 h of exposure to hypoxia (c). Western blot of HIF3α in IPF derived fibroblasts after treatment with 2 and 5uM of 5-Aza-2′-deoxycytidine (5-Aza) for 5 days. β-actin was used as a loading control (d). Each bar represents the mean ± SD of the two experiments (e)